An Allosteric Transcription Factor DNA-Binding Electrochemical Biosensor for Progesterone.
Karthika SankarR BaerChloé GrazonRobert C SabatelleSébastien LecommandouxCatherine M KlapperichJames E GalaganMark W GrinstaffPublished in: ACS sensors (2022)
We describe an electrochemical strategy to transduce allosteric transcription factor (aTF) binding affinity to sense steroid hormones. Our approach utilizes square wave voltammetry to monitor changes in current output as a progesterone (PRG)-specific aTF (SRTF1) unbinds from the cognate DNA sequence in the presence of PRG. The sensor detects PRG in artificial urine samples with sufficient sensitivity suitable for clinical applications. Our results highlight the capability of using aTFs as the biorecognition elements to develop electrochemical point-of-care biosensors for the detection of small-molecule biomarkers and analytes.
Keyphrases
- transcription factor
- dna binding
- label free
- small molecule
- gold nanoparticles
- protein protein
- molecularly imprinted
- ionic liquid
- genome wide identification
- estrogen receptor
- single molecule
- cell free
- circulating tumor
- endoplasmic reticulum stress
- mass spectrometry
- oxidative stress
- capillary electrophoresis
- heat shock protein
- simultaneous determination
- amino acid