Antibacterial Multi-Layered Nanocellulose-Based Patches Loaded with Dexpanthenol for Wound Healing Applications.
Daniela F S FonsecaJoão P F CarvalhoVeronica Isabel Correia BastosHelena OliveiraCatarina MoreirinhaAdelaide AlmeidaArmando J D SilvestreCarla VilelaCarmen S R FreirePublished in: Nanomaterials (Basel, Switzerland) (2020)
Antibacterial multi-layered patches composed of an oxidized bacterial cellulose (OBC) membrane loaded with dexpanthenol (DEX) and coated with several chitosan (CH) and alginate (ALG) layers were fabricated by spin-assisted layer-by-layer (LbL) assembly. Four patches with a distinct number of layers (5, 11, 17, and 21) were prepared. These nanostructured multi-layered patches reveal a thermal stability up to 200 °C, high mechanical performance (Young's modulus ≥ 4 GPa), and good moisture-uptake capacity (240-250%). Moreover, they inhibited the growth of the skin pathogen Staphylococcus aureus (3.2-log CFU mL-1 reduction) and were non-cytotoxic to human keratinocytes (HaCaT cells). The in vitro release profile of DEX was prolonged with the increasing number of layers, and the time-dependent data imply a diffusion/swelling-controlled drug release mechanism. In addition, the in vitro wound healing assay demonstrated a good cell migration capacity, headed to a complete gap closure after 24 h. These results certify the potential of these multi-layered polysaccharides-based patches toward their application in wound healing.
Keyphrases
- wound healing
- cell migration
- staphylococcus aureus
- drug release
- reduced graphene oxide
- drug delivery
- transition metal
- highly efficient
- room temperature
- high throughput
- genome wide
- solar cells
- gene expression
- single cell
- big data
- gold nanoparticles
- density functional theory
- candida albicans
- artificial intelligence
- cancer therapy
- methicillin resistant staphylococcus aureus
- human health
- dna methylation
- machine learning
- escherichia coli
- pseudomonas aeruginosa
- cystic fibrosis
- data analysis
- deep learning
- biofilm formation