Mitochondria play a key role in the cerebral ischemia-reperfusion injury. Although the extracellular signal-regulated kinase 1/2 inhibitor PD98059 (PD) is a selective and reversible flavonoid that can protect the mitochondria in a rat model of cardiac arrest/cardiopulmonary resuscitation, its role requires further confirmation. In this study, we investigated whether PD could maintain mitochondrial homeostasis and decrease reactive oxygen species (ROS) production in neuroblastoma (SH-SY5Y) cells exposed to oxygen-glucose deprivation/reperfusion (OGD/R). PD improved the mitochondrial morphology and function, reversed the increase in ROS production and cell apoptosis, and reduced total-superoxide dismutase and Mn-superoxide dismutase activities induced by OGD/R. PD decreases ROS production and improves mitochondrial morphology and function, protecting SH-SY5Y cells against OGD/R-induced injury.
Keyphrases
- oxidative stress
- reactive oxygen species
- induced apoptosis
- cardiac arrest
- cardiopulmonary resuscitation
- cell death
- cell cycle arrest
- ischemia reperfusion injury
- dna damage
- diabetic rats
- acute myocardial infarction
- endoplasmic reticulum stress
- signaling pathway
- cerebral ischemia
- cell proliferation
- type diabetes
- heart failure
- acute coronary syndrome
- metabolic syndrome
- adipose tissue
- endothelial cells
- subarachnoid hemorrhage
- mass spectrometry
- heat stress
- heat shock protein
- high speed