Amperometric Miniaturised Portable Enzymatic Nanobiosensor for the Ultrasensitive Analysis of a Prostate Cancer Biomarker.
Stefania HroncekovaLenka LorencovaTomas BertokMichal HiresEduard JaneMarek BučkoPeter KasakJan TkacPublished in: Journal of functional biomaterials (2023)
Screen-printing technology is a game changer in many fields including electrochemical biosensing. Two-dimensional nanomaterial MXene Ti 3 C 2 T x was integrated as a nanoplatform to immobilise enzyme sarcosine oxidase (SOx) onto the interface of screen-printed carbon electrodes (SPCEs). A miniaturised, portable, and cost-effective nanobiosensor was constructed using chitosan as a biocompatible glue for the ultrasensitive detection of prostate cancer biomarker sarcosine. The fabricated device was characterised with energy-dispersive X-ray spectroscopy (EDX), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). Sarcosine was detected indirectly via the amperometric detection of H 2 O 2 formed during enzymatic reaction. The nanobiosensor could detect sarcosine down to 7.0 nM with a maximal peak current output at 4.10 ± 0.35 × 10 -5 A using only 100 µL of a sample per measurement. The assay run in 100 μL of an electrolyte showed the first linear calibration curve in a concentration window of up to 5 μM with a slope of 2.86 μA·μM -1 , and the second linear calibration curve in the range of 5-50 μM with a slope of 0.32 ± 0.01 μA·μM -1 (R 2 = 0.992). The device provided a high recovery index of 92.5% when measuring an analyte spiked into artificial urine, and could be used for detection of sarcosine in urine for at least a period of 5 weeks after the preparation.
Keyphrases
- label free
- prostate cancer
- ionic liquid
- gold nanoparticles
- molecularly imprinted
- hydrogen peroxide
- low cost
- high resolution
- high throughput
- reduced graphene oxide
- radical prostatectomy
- photodynamic therapy
- solid state
- loop mediated isothermal amplification
- real time pcr
- single molecule
- solid phase extraction
- quantum dots
- drug delivery
- drug release
- wastewater treatment
- transcription factor
- magnetic resonance imaging
- cancer therapy
- magnetic resonance
- nitric oxide
- single cell
- preterm birth
- gestational age