Multiplex PCR for identification of two butterfly sister species: Eurema mandarina and Eurema hecabe.

Mai N MiyataDaisuke KageyamaMasashi Nomura

Published in: BMC research notes (2020)

We successfully analyzed the nucleotide sequences of the Z chromosome-linked triose phosphate isomerase (Tpi) gene to develop a multiplex polymerase chain reaction (PCR) that amplified ca. 120-bp products for E. mandarina and ca. 375-bp products for E. hecabe. We suggest that multiplex PCR using Tpi with appropriately designed primers can be used to accurately and reliably distinguish between other closely related Lepidoptera species.

Keyphrases

real time pcr
high throughput
copy number
genetic diversity
genome wide
protein kinase
gene expression
transcription factor
drug induced