Anti-inflammatory Polyketides from the Marine-Derived Fungus Eutypella scoparia .

Three new polyketides, eutyketides A and B ( 1 and 2 ) and cytosporin X ( 3 ), along with four known compounds ( 4 - 7 ), were obtained from the marine-derived fungus Eutypella scoparia . The planar structures of 1 and 2 were elucidated by extensive HRMS and 1D and 2D NMR analyses. Their relative configurations of C-13 and C-14 were determined with chemical conversions by introducing an acetonylidene group. The absolute configurations of 1 - 3 were determined by comparing their experimental electronic circular dichroism (ECD) data with their computed ECD results. All of the isolated compounds were tested for their anti-inflammatory activities on lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophages. Compounds 5 and 6 showed stronger anti-inflammatory activities than the other compounds, with the inhibition of 49.0% and 54.9% at a concentration of 50.0 µg/mL, respectively.