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The phage-encoded PIT4 protein affects multiple two-component systems of Pseudomonas aeruginosa .

Kaat SchrovenLeena PutzeysAlison KerremansPieter-Jan CeyssensMarta VallinoJan PaeshuyseFarhana HaqueAhmed YusufMatthias D KochRob Lavigne
Published in: Microbiology spectrum (2023)
ranks in the top three of pathogens for which the development of novel antibiotics is the most crucial. The pathogen causes both acute and chronic infections, especially in patients who are the most vulnerable. Therefore, efforts are urgently needed to develop alternative therapies. One path explored in this article is the use of bacteriophages and, more specifically, phage-derived proteins. In this study, a phage-derived protein was studied that impacts key virulence factors of the pathogen via interaction with multiple histidine kinases of TCSs. The fundamental insights gained for this protein can therefore serve as inspiration for the development of an anti-virulence compound that targets the bacterial TCS.
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