Azanone (HNO): generation, stabilization and detection.
Cecilia Mariel GallegoAgostina MazzeoPaola VargasSebastián SuárezJuan PellegrinoFabio DoctorovichPublished in: Chemical science (2021)
HNO (nitroxyl, azanone), joined the 'biologically relevant reactive nitrogen species' family in the 2000s. Azanone is impossible to store due to its high reactivity and inherent low stability. Consequently, its chemistry and effects are studied using donor compounds, which release this molecule in solution and in the gas phase upon stimulation. Researchers have also tried to stabilize this elusive species and its conjugate base by coordination to metal centers using several ligands, like metalloporphyrins and pincer ligands. Given HNO's high reactivity and short lifetime, several different strategies have been proposed for its detection in chemical and biological systems, such as colorimetric methods, EPR, HPLC, mass spectrometry, fluorescent probes, and electrochemical analysis. These approaches are described and critically compared. Finally, in the last ten years, several advances regarding the possibility of endogenous HNO generation were made; some of them are also revised in the present work.
Keyphrases
- label free
- mass spectrometry
- living cells
- gold nanoparticles
- loop mediated isothermal amplification
- high performance liquid chromatography
- real time pcr
- ms ms
- liquid chromatography
- fluorescent probe
- simultaneous determination
- high resolution
- sensitive detection
- cancer therapy
- ionic liquid
- capillary electrophoresis
- nitric oxide
- genetic diversity
- drug discovery
- molecularly imprinted
- photodynamic therapy
- data analysis