Identification of a Candidate Gene Set Signature for the Risk of Progression in IgM MGUS to Smoldering/Symptomatic Waldenström Macroglobulinemia (WM) by a Comparative Transcriptome Analysis of B Cells and Plasma Cells.
Alessandra TrojaniBarbara Di CamilloLuca Emanuele BossiLivia LeuzziAntonino GrecoAlessandra TedeschiAnna Maria FrustaciMarina DeodatoGiulia ZamprognaAlessandro BeghiniRoberto CairoliPublished in: Cancers (2021)
Waldenström Macroglobulinemia (WM) is a B-cell lymphoma characterized by the precursor condition IgM monoclonal gammopathies of undetermined significance (IgM MGUS). We performed a gene expression profiling study to compare the transcriptome signatures of bone marrow (BM) B-cells and plasma cells of 36 WM patients, 13 IgM MGUS cases, and 7 healthy subjects used as controls (CTRLs) by Affymetrix microarray. We determined 2038 differentially expressed genes (DEGs) in CD19+ cells and 29 DEGs genes in CD138+ cells, respectively. The DEGs identified in B-cells were associated with KEGG pathways, mainly involved in hematopoietic cell lineage antigens, cell adhesion/focal adhesion/transmembrane proteins, adherens junctions, Wnt-signaling pathway, BCR-signaling pathway, calcium signaling pathway, complement/coagulation cascade, platelet activation, cytokine-cytokine receptor interactions, and signaling pathways responsible for cell cycle, apoptosis, proliferation and survival. In conclusion, we showed the deregulation of groups of genes belonging to KEGG pathways in the comparison among WM vs. IgM MGUS vs. CTRLs in B-cells. Interestingly, a small set of genes in B-cells displayed a common transcriptome expression profile between WM and IgM MGUS compared to CTRLs, suggesting its possible role in the risk of transformation of IgM MGUS to WM.
Keyphrases
- induced apoptosis
- signaling pathway
- genome wide
- cell cycle arrest
- pi k akt
- endoplasmic reticulum stress
- bone marrow
- genome wide identification
- cell cycle
- single cell
- cell death
- bioinformatics analysis
- dna methylation
- cell proliferation
- gene expression
- stem cells
- rna seq
- acute lymphoblastic leukemia
- mesenchymal stem cells
- diffuse large b cell lymphoma
- dendritic cells
- newly diagnosed
- genome wide analysis
- multiple myeloma
- escherichia coli
- cell fate