Effective production of human growth factors in Escherichia coli by fusing with small protein 6HFh8.
Young Su KimHye-Jeong LeeMan-Ho HanNam-Kyung YoonYeu-Chun KimJung-Oh AhnPublished in: Microbial cell factories (2021)
We demonstrated that 6HFh8-GF fusion proteins could be overexpressed on a g/l scale in the cytoplasm of E. coli, with the GFs subsequently highly purified and maintaining their biological activity. Hence, the small protein 6HFh8 can be used for efficient mass-production of various GFs.