Single Cell Map of Human Azoospermia Testis Caused by Cyclophosphamide Chemotherapy.
Jian CaoXueheng ZhaoZailong QinShanshan LvLin DuZhizhong LiuLiqing FanHao BoPublished in: Scientific data (2024)
Chemotherapeutic drugs will affect the process of spermatogenesis. However, most current studies on the effects of chemotherapeutic drugs on spermatogenesis are based on mouse models, with a shortage of human body evidence. In addition, the mechanism of chemotherapeutic drugs causing spermatogenesis disorder is not clear. Therefore, we have collected the testicular tissues of an inguinal-lipoma patient whose testes were resected after chemotherapy and a patient who had normal spermatogenesis disorder and underwent single-nucleus RNA sequencing (snRNA-Seq). After quality control, we obtained a total of 27,957 high-quality cells, including 18,612 normal cells and 9,345 drug-treated cells, which were all used in analyzing the mechanism of chemotherapeutic drugs causing spermatogenesis disorder. This study has provided data resources and references for exploring the mechanism of chemotherapeutic drugs causing spermatogenesis disorder with the insight of protecting the spermatogenic abilities of male tumor patients receiving chemotherapy.
Keyphrases
- induced apoptosis
- single cell
- cell cycle arrest
- endothelial cells
- quality control
- rna seq
- endoplasmic reticulum stress
- locally advanced
- cell death
- low dose
- signaling pathway
- prostate cancer
- lymph node
- emergency department
- cell proliferation
- induced pluripotent stem cells
- high throughput
- big data
- electronic health record
- adverse drug
- chemotherapy induced
- high density