Detecting M-Protein via Mass Spectrometry and Affinity Beads: Enrichment With Mixed Kappa-Lambda Beads Enables Prompt Application in Clinical Laboratories.

Jikyo Lee Jung Hoon Choi Eun-Hee Kim Jihyun Im Heeyoun Hwang Seojin Yang Joon Hee Lee Kyunghoon Lee Junghan Song Seungman Park Sang Hoon Song

Published in: Annals of laboratory medicine (2024)

NBs for M-protein detection, particularly with mixed kappa-lambda beads, identified monoclonal peaks with both MALDI-TOF and ESI-qTOF analyses. Qualitative analysis using MALDI-TOF yielded results comparable with that of IFE. NB-MALDI-TOF might be used as an alternative method to replace conventional tests (such as IFE) to detect M-protein with high sensitivity.

Keyphrases

mass spectrometry
ms ms
liquid chromatography
capillary electrophoresis
high performance liquid chromatography
gas chromatography
high resolution
nuclear factor
protein protein
amino acid
binding protein
small molecule
systematic review
inflammatory response
label free