A Portable Nucleic Acid Testing Platform with Photosensitization, a Three-Dimensionally Printed Multipiece Chip, and Digital Color Sensing.
Tianyu DongRuolin DingRongxing ZhouChenlan ShenWill SheridanDixon PaezZhi-He ZhaoPeng WuFeng LiPublished in: Analytical chemistry (2024)
Portable nucleic acid testing (NAT) holds great promise for point-of-care disease diagnosis and field-based applications but remains difficult to achieve. Herein, we describe a portable NAT that streamlines loop-mediated isothermal amplification with photosensitization-based color development in a fully sealed 3D-printed multipiece chip. Using a smartphone accessory and an APP, we also introduce a calibration-free quantification approach via digital color sensing and library matching. With these innovative approaches, our detection platform is highly accessible, allowing for rapid and sensitive NAT without requiring sophisticated instruments and well-trained personnel. The field applicability of our NAT platform was demonstrated by detecting tuberculosis infections in clinical sputum samples and food adulteration in commercial salmon meat products.
Keyphrases
- nucleic acid
- loop mediated isothermal amplification
- high throughput
- low cost
- sensitive detection
- mycobacterium tuberculosis
- pulmonary tuberculosis
- circulating tumor cells
- cystic fibrosis
- single cell
- big data
- machine learning
- emergency department
- hiv aids
- resistance training
- deep learning
- artificial intelligence
- patient reported outcomes
- human immunodeficiency virus
- human health
- real time pcr
- antiretroviral therapy
- adverse drug