The Autophagic and Apoptotic Death of Forebrain Neurons of Rats with Global Brain Ischemia Is Diminished by the Intranasal Administration of Insulin: Possible Mechanism of Its Action.
Irina O ZakharovaLiubov V BayunovaDaria K AvrovaAlina D TretyakovaAlexander O ShpakovNatalia F AvrovaPublished in: Current issues in molecular biology (2024)
Insulin is a promising neuroprotector. To better understand the mechanism of insulin action, it was important to show its ability to diminish autophagic neuronal death in animals with brain ischemic and reperfusion injury. In forebrain ischemia and reperfusion, the number of live neurons in the hippocampal CA1 region and frontal cortex of rats decreased to a large extent. Intracerebroventricular administration of the autophagy and apoptosis inhibitors to ischemic rats significantly increased the number of live neurons and showed that the main part of neurons died from autophagy and apoptosis. Intranasal administration of 0.5 IU of insulin per rat (before ischemia and daily during reperfusion) increased the number of live neurons in the hippocampal CA1 region and frontal brain cortex. In addition, insulin significantly diminished the level of autophagic marker LC3B-II in these forebrain regions, which markedly increased during ischemia and reperfusion. Our studies demonstrated for the first time the ability of insulin to decrease autophagic neuronal death, caused by brain ischemia and reperfusion. Insulin administered intranasally activated the Akt-kinase (activating the mTORC1 complex, which inhibits autophagy) and inhibited the AMP-activated protein kinase (which activates autophagy) in the hippocampus and frontal cortex of rats with brain ischemia and reperfusion.
Keyphrases
- cerebral ischemia
- cell death
- type diabetes
- subarachnoid hemorrhage
- blood brain barrier
- brain injury
- cell cycle arrest
- functional connectivity
- protein kinase
- endoplasmic reticulum stress
- oxidative stress
- glycemic control
- resting state
- signaling pathway
- spinal cord
- acute myocardial infarction
- working memory
- cell proliferation
- insulin resistance
- mass spectrometry
- skeletal muscle
- acute ischemic stroke
- coronary artery disease
- pi k akt
- metabolic syndrome
- cognitive impairment