Multilayered Porous Titanium-Based 3rd Generation Biomaterial Designed for Endosseous Implants.
George Calin DindeleganAlexandra CaziucIoana BrieOlga SoritauMaximilian George DindeleganVasile BintintanVioleta PascalauCarmen MihuCatalin PopaPublished in: Materials (Basel, Switzerland) (2021)
This work proposes a novel complex multi-layered material consisting of porous titanium as a substrate and a complex coating consisting of a chitosan film engulfing microsphere loaded with growth factors such as BMP2 (bone morphogenic protein 2) and IGF1 (insulin-like growth factor-1). The microspheres were obtained through deposition of dual layers of calcium cross linked pectin-chitosan/pectin polyelectrolyte onto a BSA (bovine serum albumin) gel core. The multilayer was conceived to behave like a 3rd generation biomaterial, by slow delivery of viable growth factors around implants, and to assist the healing of implantation wound and the development of new vital bone. The biologic effect of the delivery of growth factors was studied in vitro, on MSC-CD1 mesenchymal stem cells, and in vivo, on CD1 mice. Proliferation and differentiation of cells were accelerated by growth factors, especially IGF1 for proliferation and BMP2 for differentiation. In vivo tests analyzed histologically and by MicroCT show a more structured tissue around BMP2 samples. The present concept will give the best clinical results if both growth factors are delivered together by a coating film that contains a double population of microcarriers.
Keyphrases
- mesenchymal stem cells
- bone regeneration
- wound healing
- drug delivery
- soft tissue
- umbilical cord
- signaling pathway
- tissue engineering
- induced apoptosis
- reduced graphene oxide
- pi k akt
- bone mineral density
- growth hormone
- binding protein
- cell cycle arrest
- hyaluronic acid
- highly efficient
- bone marrow
- room temperature
- bone loss
- metal organic framework
- rheumatoid arthritis
- cell therapy
- amino acid
- high fat diet induced
- cancer therapy
- type diabetes
- protein protein
- cell wall
- body composition
- adipose tissue
- gold nanoparticles
- high resolution