Watsonianone A from Rhodomyrtus tomentosa Fruit Attenuates Respiratory-Syncytial-Virus-Induced Inflammation In Vitro.
Ling ZhuangLi-Feng ChenYu-Bo ZhangZhong LiuXu-Hui XiaoWei TangGuo-Cai WangWen-Jun SongYao-Lan LiMan-Mei LiPublished in: Journal of agricultural and food chemistry (2017)
Respiratory syncytial virus (RSV) is one of the most common respiratory pathogens. Immoderate inflammation plays a great role in causing RSV-induced diseases. In the present study, watsonianone A, isolated from the fruit of Rhodomyrtus tomentosa (Ait.) Hassk, was found to show a good inhibitory effect on RSV-induced NO production, with a half-maximal inhibitory concentration of 37.2 ± 1.6 μM. Enzyme-linked immunosorbent assay and fluorescence quantitative polymerase chain reaction analyses indicated that watsonianone A markedly reduced both mRNA and protein levels of tumor necrosis factor α, interleukin 6, and monocyte chemoattractant protein 1 in RSV-infected RAW264.7 cells. Mechanistically, watsonianone A inhibited nuclear factor κB (NF-κB) activation by suppressing IκBα phosphorylation. Further analysis revealed that watsonianone A activated the thioredoxin system and decreased intracellular reactive oxygen species (ROS) levels, which are closely associated with NF-κB activation in RSV-infected cells. These results reveal that watsonianone A can attenuate RSV-induced inflammation via the suppression of ROS-sensitive inflammatory signaling.
Keyphrases
- respiratory syncytial virus
- oxidative stress
- diabetic rats
- nuclear factor
- reactive oxygen species
- high glucose
- induced apoptosis
- respiratory tract
- signaling pathway
- dna damage
- cell death
- high throughput
- single cell
- heart rate
- binding protein
- gene expression
- mass spectrometry
- pi k akt
- quantum dots
- high intensity
- antimicrobial resistance