Lack of specificity associated with using molecular beacons in loop mediated amplification assays.
Patrick HardingeJames A H MurrayPublished in: BMC biotechnology (2019)
Molecular beacons can be used to detect LAMP amplification but do not provide sequence specificity. The method can be used to determine effectively LAMP amplification from other primer-driven events, but does not discriminate between different LAMP amplicons. It is therefore unsuitable for multiplex LAMP reactions due to non-specific detection of LAMP amplification.