RuCu Nanosheets with Ultra-high Nanozyme Activity for Chemodynamic Therapy.

Nanoenzymes have been widely explored for chemodynamic therapy (CDT) in cancer treatment. However, poor catalytic efficiency of nanoenzymes, especially in the tumor microenvironment with insufficient H 2 O 2 and mild acidity, limits the effect of CDT. Herein, we developed a new ultrathin RuCu nanosheet (NS) based nanoenzyme which has a large specific surface area and abundant channels and defects. The RuCu NSs showed superb catalytic efficiency for the oxidation of peroxidase substrate H 2 O 2 at a wide range of pH and their catalytic efficiency (k cat /K m = 177.2 M -1 s -1 ) is about 14.9 times higher than that of the single-atom catalyst FeN 3 P. Besides an efficient nanozyme as peroxidase, the RuCu NSs possessed other two enzyme activities, not only disproportionating superoxide anion to produce H 2 O 2 but also consuming GSH to keep a high concentration of H 2 O 2 in the tumor microenvironment for Fenton reaction. With these advantages, the RuCu NSs exhibited good performance to kill cancer cells and inhibit tumor growth in mice, demonstrating a promising potential as new CDT reagent. This article is protected by copyright. All rights reserved.