Untargetted Metabolomic Exploration of the Mycobacterium tuberculosis Stress Response to Cinnamon Essential Oil.
Elwira SieniawskaRafał SawickiJoanna GolusMilen I GeorgievPublished in: Biomolecules (2020)
The antimycobacterial activity of cinnamaldehyde has already been proven for laboratory strains and for clinical isolates. What is more, cinnamaldehyde was shown to threaten the mycobacterial plasma membrane integrity and to activate the stress response system. Following promising applications of metabolomics in drug discovery and development we aimed to explore the mycobacteria response to cinnamaldehyde within cinnamon essential oil treatment by untargeted liquid chromatography-mass spectrometry. The use of predictive metabolite pathway analysis and description of produced lipids enabled the evaluation of the stress symptoms shown by bacteria. This study suggests that bacteria exposed to cinnamaldehyde could reorganize their outer membrane as a physical barrier against stress factors. They probably lowered cell wall permeability and inner membrane fluidity, and possibly redirected carbon flow to store energy in triacylglycerols. Being a reactive compound, cinnamaldehyde may also contribute to disturbances in bacteria redox homeostasis and detoxification mechanisms.
Keyphrases
- essential oil
- mass spectrometry
- liquid chromatography
- mycobacterium tuberculosis
- drug discovery
- cell wall
- high resolution mass spectrometry
- tandem mass spectrometry
- high resolution
- stress induced
- gas chromatography
- capillary electrophoresis
- escherichia coli
- high performance liquid chromatography
- simultaneous determination
- mental health
- endothelial cells
- replacement therapy
- solid phase extraction
- pulmonary tuberculosis
- combination therapy
- depressive symptoms