A tunable fluorescent probe for superoxide anion detection during inflammation caused by Treponema pallidum .

Syphilis, caused by Treponema pallidum ( T. pallidum ), is associated with the oxidative stress due to its inflammation-like symptom, and detecting the reactive oxygen species (ROS) is crucial for monitoring the infectious process. Herein, we design and synthesize a perylene-based tunable fluorescent probe, PerqdOH, which can detect endogenous O 2 ˙ - during T. pallidum infection. The fluorescence peak shifted from 540 nm to 750 nm with increasing O 2 ˙ - levels. Besides, both decreased green fluorescence and enhanced red fluorescence could be observed simultaneously during the in vitro infection, providing the real-time monitoring of intracellular O 2 ˙ - caused by T. pallidum . Furthermore, the probe exhibited a remarkable signal in the treponemal lesions on the back of a rabbit model. Taken together, our synthesized PerqdOH holds great potential for application in clarifying the infectious process caused by T. pallidum in real time.