Sequential Time-Kill, a Simple Experimental Trick To Discriminate between Pharmacokinetics/Pharmacodynamics Models with Distinct Heterogeneous Subpopulations versus Homogenous Population with Adaptive Resistance.
A ChauzyH IhM JacobsSandrine MarchandN GrégoireWilliam CouetJulien M BuyckPublished in: Antimicrobial agents and chemotherapy (2020)
Experiments were conducted with polymyxin B and two Klebsiella pneumonia isogenic strains (the wild type, KP_WT, and its transconjugant carrying the mobile colistin resistance gene, KP_MCR-1) to demonstrate that conducting two consecutive time-kill experiments (sequential TK) represents a simple approach to discriminate between pharmacokinetics/pharmacodynamics models with two heterogeneous subpopulations or adaptive resistance.
Keyphrases
- escherichia coli
- wild type
- multidrug resistant
- klebsiella pneumoniae
- gram negative
- drug resistant
- pseudomonas aeruginosa
- copy number
- acinetobacter baumannii
- gene expression
- intensive care unit
- transcription factor
- cystic fibrosis
- acute respiratory distress syndrome
- extracorporeal membrane oxygenation
- mechanical ventilation