Cell biologists have long sought the ability to observe intracellular structures in living cells without labels. This study presents procedures to adjust a commercially available apodized phase-contrast (APC) microscopy system for better visualizing the dynamic behaviors of various subcellular organelles in living cells. By harnessing the versatility of this technique to capture sequential images, we could observe morphological changes in cellular geometry after virus infection in real time without probes or invasive staining. The tune-up APC microscopy system is a highly efficient platform for simultaneously observing the dynamic behaviors of diverse subcellular structures with exceptional resolution.
Keyphrases
- living cells
- high resolution
- single molecule
- label free
- highly efficient
- fluorescent probe
- magnetic resonance
- high speed
- mass spectrometry
- high throughput
- optical coherence tomography
- single cell
- cell therapy
- deep learning
- stem cells
- contrast enhanced
- reactive oxygen species
- big data
- magnetic resonance imaging
- flow cytometry
- bone marrow