High-Throughput Single-Cell Sequencing of both TCR-β Alleles.

Tomonori HosoyaHongyang LiChia-Jui KuQingqing WuYuanfang GuanJames Douglas Engel

Published in: Journal of immunology (Baltimore, Md. : 1950) (2018)

Allelic exclusion is a vital mechanism for the generation of monospecificity to foreign Ags in B and T lymphocytes. In this study, we developed a high-throughput barcoded method to simultaneously analyze the VDJ recombination status of both mouse TCR-β alleles in hundreds of single cells using next-generation sequencing.

Keyphrases

high throughput
single cell
rna seq
regulatory t cells
induced apoptosis
cell cycle arrest
dna damage
dna repair
copy number
immune response
oxidative stress
endoplasmic reticulum stress
signaling pathway
dendritic cells
pi k akt