In Vitro Toxicity Study of a Porous Iron(III) Metal‒Organic Framework.

Gongsen ChenXin LengJuyuan LuoLongtai YouChanghai QuXiaoxv DongHongliang HuangXingbin YinJian Ni

Published in: Molecules (Basel, Switzerland) (2019)

A MIL series metal‒organic framework (MOF), MIL-100(Fe), was successfully synthesized at the nanoscale and fully characterized by TEM, TGA, XRD, FTIR, DLS, and BET. A toxicological assessment was performed using two different cell lines: human normal liver cells (HL-7702) and hepatocellular carcinoma (HepG2). In vitro cytotoxicity of MIL-100(Fe) was evaluated by the MTT assay, LDH releasing rate assay, DAPI staining, and annexin V/PI double staining assay. The safe dose of MIL-100(Fe) was 80 μg/mL. It exhibited good biocompatibility, low cytotoxicity, and high cell survival rate (HL-7702 cells' viability >85.97%, HepG2 cells' viability >91.20%). Therefore, MIL-100(Fe) has a potential application as a drug carrier.

Keyphrases

metal organic framework
induced apoptosis
high throughput
cell cycle arrest
endothelial cells
endoplasmic reticulum stress
oxidative stress
cell death
signaling pathway
flow cytometry
pi k akt
climate change
high resolution
drug induced
cell proliferation
adverse drug
electronic health record