Identification of pyridoxal phosphate-modified proteins using mass spectrometry.
Yue WuJin ChenZheyi LiuFang-Jun WangPublished in: Rapid communications in mass spectrometry : RCM (2018)
MSA-assisted CID was used to provide better identification of PLP-modified peptides. Furthermore, tryptophanase with PLP modification at K270 in E. coli was identified with Ti4+ -IMAC enrichment followed by ALP treatment. This method provides a promising alternative for investigating biological functions of PLP-modified proteins.