A novel method for miRNA detection based on target-triggered transcription of a light-up RNA aptamer.

Seoyoung LeeHansol KimYeonkyung ParkHyun Gyu Park

Published in: Chemical communications (Cambridge, England) (2022)

We herein describe a novel method for miRNA detection based on target-triggered transcription of a light-up RNA aptamer (TTRApt), consequently producing a highly enhanced fluorescence signal through specific binding of a light-up RNA aptamer to the corresponding dye. Using this strategy, we successfully identified target miRNA down to 59.4 aM with excellent specificity.

Keyphrases

label free
gold nanoparticles
sensitive detection
loop mediated isothermal amplification
magnetic nanoparticles
transcription factor
nucleic acid
real time pcr
quantum dots