Phytochemical investigation of Nigrospora zimmermanii isolated from Poincianella pluviosa (Sibipiruna): metabolites characterisation and screening for anti-inflammatory activity.
Miller Santos FerreiraAlbert Katchborian NetoKaren de Jesus NicácioMário Ferreira Conceição SantosDanielle Ferreira DiasDaniela Aparecida Chagas de PaulaMarisi Gomes SoaresPublished in: Natural product research (2024)
Endophytic fungi residing symbiotically in plant tissues are promising sources of bioactive natural products. This study explored the anti-inflammatory potential of an endophytic fungus isolated from the Brazilian medicinal plant Poincianella pluviosa (Sibipiruna). The extract from the endophyte FPD13 exhibited potential ex vivo anti-inflammatory effects by inhibiting prostaglandin E 2 (PGE 2 ) release by 75.22%. Phytochemical analysis using High-Performance Liquid Chromatography (HPLC), Nuclear Magnetic Resonance (NMR), and Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS) enabled the isolation and identification of three compounds, including the macrolide Nigrosporolide, the phenyl-propanol Tyrosol, and the terpene Decarestrictine A. Morphological characteristics and Internal Transcribed Spacers region (ITS) sequencing classified fungus FPD13 as Nigrospora zimmermanii . The results reveal the anti-inflammatory potential and chemical diversity of P. pluviosa endophytes, warranting further investigation into the bioactivity and structure elucidation of their bioactive metabolites.
Keyphrases
- high resolution mass spectrometry
- liquid chromatography
- tandem mass spectrometry
- high performance liquid chromatography
- mass spectrometry
- simultaneous determination
- ultra high performance liquid chromatography
- anti inflammatory
- magnetic resonance
- solid phase extraction
- ms ms
- gas chromatography
- high resolution
- oxidative stress
- single cell
- signaling pathway
- gene expression
- risk assessment
- computed tomography
- climate change
- dna methylation
- solid state
- tissue engineering