Selective interactions of Co 2+ -Ca 2+ -concanavalin A with high mannose N -glycans.

Yunha HwangJae-Hee JeongDong-Heon LeeSeung Jae Lee

Published in: Dalton transactions (Cambridge, England : 2003) (2023)

Concanavalin A (ConA) has an intrinsic binding affinity to carbohydrates. Here, we obtained Co 2+ -Ca 2+ -ConA (2.83 Å, PDB: 8I7Q) via X-ray crystallography by substituting native ConA (Mn 2+ -Ca 2+ ); it has binding selectivity for high-mannose N -glycan similar to native ConA. Our findings may thus inform antiviral reagent design.

Keyphrases

protein kinase
high resolution
dna binding
cell surface
binding protein
computed tomography
room temperature
dual energy
structural basis