A multicomponent reaction platform towards multimodal near-infrared BODIPY dyes for STED and fluorescence lifetime imaging.
Antonio FernándezNicola KiellandAshraff MakdaNeil O CarragherM Carmen Gonzalez-GarciaLaura Espinar-BarrancoJuan A González-VeraÁngel OrteRodolfo LavillaMarc VendrellPublished in: RSC chemical biology (2022)
We report a platform combining multicomponent reaction synthesis and automated cell-based screening to develop biocompatible NIR-BODIPY fluorophores. From a library of over 60 fluorophores, we optimised compound NIRBD-62c as a multimodal probe with suitable properties for STED super-resolution and fluorescence lifetime imaging. Furthermore, we employed NIRBD-62c for imaging trafficking inside cells and to examine how pharmacological inhibitors can alter the vesicular traffic between intracellular compartments and the plasma membrane.
Keyphrases
- high resolution
- high throughput
- fluorescent probe
- induced apoptosis
- single molecule
- single cell
- fluorescence imaging
- machine learning
- pain management
- photodynamic therapy
- mesenchymal stem cells
- endoplasmic reticulum stress
- drug release
- oxidative stress
- bone marrow
- signaling pathway
- electron transfer
- pi k akt
- aqueous solution