SPR-Based Detection of ASF Virus in Cells.
Alessandro CapoAlessia CalabreseMaciej Piotr FrantMarek WalczakSusan SzathmaryGeorgios ManessisIoannis BossisMaria StaianoSabato D'AuriaAntonio VarrialePublished in: International journal of molecular sciences (2022)
African swine fever (ASF) is one of the most dangerous hemorrhagic infectious diseases that affect domestic and wild pigs. Currently, neither a vaccine nor effective treatments are available for this disease. As regards the degree of virulence, ASFV strains can be divided into high, moderate, or low virulence. The main detection methods are based on the use of the polymerase chain reaction (PCR). In order to prevent an uncontrolled spread of ASF, new on-site techniques that can enable the identification of an early-stage disease are needed. We have developed a specific immunological SPR-based assay for ASFV antigen detection directly in liquid samples. The developed assay allows us to detect the presence of ASFV at the dose of 10 3 HAD 50 / mL .
Keyphrases
- escherichia coli
- early stage
- real time pcr
- infectious diseases
- loop mediated isothermal amplification
- pseudomonas aeruginosa
- staphylococcus aureus
- label free
- induced apoptosis
- biofilm formation
- antimicrobial resistance
- high intensity
- cystic fibrosis
- ionic liquid
- endoplasmic reticulum stress
- sensitive detection
- bioinformatics analysis
- pi k akt