Piperine synergistically enhances the effect of temozolomide against temozolomide-resistant human glioma cell lines.
Somi JeongSeunghwa JungGyun-Seok ParkJuhyun ShinJae-Wook OhPublished in: Bioengineered (2021)
Temozolomide (TMZ) is an alkylating chemotherapy agent used in the clinical treatment of glioblastoma multiforme (GBM) patients. Piperine (PIP) is a naturally occurring pungent nitrogenous substance present in the fruits of peppers. We investigated the anti-cancer efficacies of PIP alone and in combination with TMZ in GBM cellsusingparameters such as cell proliferation, cellular apoptosis,caspase-8/-9/-3 activities, cell cycle kinetics, wound-healing ability, and loss of mitochondrial membrane potential (MMP). Treatment with PIP and alow concentration of PIP-TMZ, inhibited cell growth, similar to TMZ.PIP-TMZ promoted apoptosis by activation of caspase-8/-9/-3, MMP loss, and inhibition of in vitro wound-healing motility. Reverse transcription polymerase chain reaction analysis showed significant inhibition of Cyclin-dependent kinases (CDK)4/6-cyclin D and CDK2-cyclin-E expression upon treatment with a low concentration PIP-TMZ, suggesting an S to G1 arrest. Our findings provide insight into the apoptotic potential of the combination of a low concentration of PIP-TMZ, though further in vivo study will be needed for its validation.
Keyphrases
- cell cycle
- cell proliferation
- cell death
- newly diagnosed
- oxidative stress
- cell cycle arrest
- wound healing
- endoplasmic reticulum stress
- end stage renal disease
- transcription factor
- risk assessment
- mass spectrometry
- induced apoptosis
- human health
- radiation therapy
- pseudomonas aeruginosa
- cystic fibrosis
- cell migration
- binding protein
- long non coding rna
- climate change
- induced pluripotent stem cells