Zinc complexation improves angiotensin II receptor type 1 blockade and in vivo antihypertensive activity of telmisartan.
Valeria R MartínezMaría V AguirreJuan S TodaroAugusto Martins LimaNikolaos StergiopulosEvelina G FerrerPatricia A M WilliamsPublished in: Future medicinal chemistry (2020)
Background: Angiotensin II receptor blockers were designed as therapeutic agents to block the binding site of the angiotensin II receptor type 1 (AT1R). Methodology: The structure of telmisartan was modified by coordination to the biometal Zn(II), resulting in the compound ZnTelm. Its antihypertensive activity and cellular mechanisms in comparison to telmisartan were studied. Results: Compared with telmisartan, ZnTelm displayed stronger binding to AT1R (binding studies on AT1R-transfected human embryonic kidney cells) and a greater reduction of reactive oxygen species and cytosolic calcium concentration induced by angiotensin II. The antihypertensive activity of the complex (assessed in an N(G)-Nitro-L-arginine methyl ester-induced hypertension model) was significantly higher. ZnTelm also reduced hypertrophy in aortic artery rings and tubular collagen deposition. Conclusion: ZnTelm enhances the AT1R blockade and consequently its antihypertensive effect.
Keyphrases
- angiotensin ii
- blood pressure
- angiotensin converting enzyme
- vascular smooth muscle cells
- hypertensive patients
- reactive oxygen species
- endothelial cells
- high glucose
- induced apoptosis
- binding protein
- heavy metals
- pulmonary artery
- risk assessment
- oxidative stress
- cell proliferation
- left ventricular
- pulmonary hypertension
- transcription factor
- drug induced
- oxide nanoparticles