Accurate Extraction of Nanometer Distances in Multimers by Pulse EPR.

Silvia ValeraKatrin AckermannChristos PliotasHexian HuangJames H Naismith Bela Ernest Bode

Published in: Chemistry (Weinheim an der Bergstrasse, Germany) (2016)

Pulse electron paramagnetic resonance (EPR) is gaining increasing importance in structural biology. The PELDOR (pulsed electron-electron double resonance) method allows extracting distance information on the nanometer scale. Here, we demonstrate the efficient extraction of distances from multimeric systems such as membrane-embedded ion channels where data analysis is commonly hindered by multi-spin effects.

Keyphrases

data analysis
energy transfer
blood pressure
solar cells
electron microscopy
high resolution
room temperature
single molecule
density functional theory
quantum dots