An efficient method for markerless mutant generation by allelic exchange in Clostridium acetobutylicum and Clostridium saccharobutylicum using suicide vectors.

Celine FoulquierChing-Ning HuangNgoc-Phuong-Thao NguyenAxel ThielTom Wilding-SteelJulie SoulaMinyeong YooArmin EhrenreichIsabelle Meynial-SallesWolfgang LieblPhilippe Soucaille

Published in: Biotechnology for biofuels (2019)

The method described in this study will be useful for functional genomic studies and for the development of industrial strains for the production of biofuels and bulk chemicals.

Keyphrases

escherichia coli
wastewater treatment
copy number
gene expression
wild type