A mouse model of weight-drop closed head injury: emphasis on cognitive and neurological deficiency.
Igor KhalinNor Laili Azua JamariNadiawati Bt Abdul RazakZubaidah Bt HasainMohd Asri Bin Mohd NorMohd Hakimi Bin Ahmad ZainudinAinsah Bt OmarRenad AlyautdinPublished in: Neural regeneration research (2016)
Traumatic brain injury (TBI) is a leading cause of death and disability in individuals worldwide. Producing a clinically relevant TBI model in small-sized animals remains fairly challenging. For good screening of potential therapeutics, which are effective in the treatment of TBI, animal models of TBI should be established and standardized. In this study, we established mouse models of closed head injury using the Shohami weight-drop method with some modifications concerning cognitive deficiency assessment and provided a detailed description of the severe TBI animal model. We found that 250 g falling weight from 2 cm height produced severe closed head injury in C57BL/6 male mice. Cognitive disorders in mice with severe closed head injury could be detected using passive avoidance test on day 7 after injury. Findings from this study indicate that weight-drop injury animal models are suitable for further screening of brain neuroprotectants and potentially are similar to those seen in human TBI.
Keyphrases
- traumatic brain injury
- body mass index
- mouse model
- severe traumatic brain injury
- weight loss
- physical activity
- early onset
- weight gain
- metabolic syndrome
- small molecule
- risk assessment
- body weight
- replacement therapy
- drug induced
- resting state
- functional connectivity
- induced pluripotent stem cells
- high fat diet induced