Mesophyll conductance response to short-term changes in pCO₂ is related to leaf anatomy and biochemistry in diverse C₄ grasses.

Mesophyll CO₂ conductance (g_m ) in C₃ species responds to short-term (minutes) changes in environment potentially due to changes in leaf anatomical and biochemical properties and measurement artefacts. Compared with C₃ species, there is less information on g_m responses to short-term changes in environmental conditions such as partial pressure of CO₂ (pCO₂ ) across diverse C₄ species and the potential determinants of these responses. Using 16 C₄ grasses we investigated the response of g_m to short-term changes in pCO₂ and its relationship with leaf anatomy and biochemistry. In general, g_m increased as pCO₂ decreased (statistically significant increase in 12 species), with percentage increases in g_m ranging from +13% to +250%. Greater increase in g_m at low pCO₂ was observed in species exhibiting relatively thinner mesophyll cell walls along with greater mesophyll surface area exposed to intercellular air spaces, leaf N, photosynthetic capacity and activities of phosphoenolpyruvate carboxylase and Rubisco. Species with greater CO₂ responses of g_m were also able to maintain their leaf water-use efficiencies (TE_i ) under low CO₂ . Our study advances understanding of CO₂ response of g_m in diverse C₄ species, identifies the key leaf traits related to this response and has implications for improving C₄ photosynthetic models and TE_i through modification of g_m .