Recombinant overexpression of dihydroneopterin aldolase catalyst potentially regulates folate-biofortification.
Dhananjay K PandeyAtul KumarJitendra S RathoreNagendra SinghBhupendra ChaudharyPublished in: Journal of basic microbiology (2017)
We aim to investigate the prospects of increased production of folate through the overexpression of heterologous dihydroneopterin aldolase catalyst. The gene encoding aldolase catalyst was cloned into an expression vector and the induced recombinant protein was purified through metal-affinity chromatography which appeared at 14 kDa position on polyacrylamide-gel. Remarkably, a periodic increase in the extracellular and intracellular folic acid concentration was observed at 4 h growth of induced recombinant DHNA samples than control in a pH-dependent manner. Maximum folate concentration was observed with at least twofold increase in induced recombinant samples at pH8.0 compared to the significant decline at 6 h growth. Consistently, heterologous overexpression of bacterial aldolase through Agrobacterium-mediated genetic transformation of tobacco led to more than 2.5-fold increase in the folate concentration in the transgenic leaves than control tissues. These data are veritable inspecting metabolic flux in both bacterial and plant systems, thus providing directions for future research on folate agri-fortification.
Keyphrases
- high glucose
- diabetic rats
- cell proliferation
- ionic liquid
- room temperature
- highly efficient
- cell free
- reduced graphene oxide
- drug induced
- current status
- gene expression
- mass spectrometry
- genome wide
- copy number
- machine learning
- dna methylation
- metal organic framework
- oxidative stress
- artificial intelligence
- gold nanoparticles
- liquid chromatography
- data analysis
- reactive oxygen species
- capillary electrophoresis
- essential oil