A tissue-specific protein purification approach in Caenorhabditis elegans identifies novel interaction partners of DLG-1/Discs large.

Selma WaaijersJavier MuñozChristian BerendsJoão J RamalhoSoenita S GoerdayalTeck Y LowAdja D Zoumaro-DjayoonMichael HoffmannThijs KoormanRoderick P TasMartin HarterinkStefanie SeelkJana KerverCasper C HoogenraadOlaf BossingerBaris TursunSander van den HeuvelAlbert J R HeckMike Boxem

Published in: BMC biology (2016)

The method we present here is able to purify protein complexes from specific tissues. We uncovered a series of DLG-1 interactors, and conclude that ATAD-3 is a biologically relevant interaction partner of DLG-1. Finally, we conclude that MAPH-1.1 is a microtubule-associated protein of the MAP1 family and a candidate neuron-specific interaction partner of DLG-1.

Keyphrases

hiv testing
protein protein
binding protein
amino acid
genome wide
small molecule