MicroRNA-Mediated Host Immune Genes Manipulation Benefits AcMNPV Proliferation in Spodoptera frugiperda .
Jie ZhangJunaid ZafarJinrong KongFei WangXuehua ShaoRuonan ZhangRui PangHan-Hong XuXiaoxia XuFengliang JinPublished in: Journal of agricultural and food chemistry (2023)
Spodoptera frugiperda is a highly destructive migratory pest that threatens various crops globally. Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is an effective biocontrol agent against lepidopteran pests. Here, we explored the molecular mechanisms underlying the immune response to AcMNPV infection in S. frugiperda . RNA-seq and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analyses identified the Toll, IMD, and apoptosis pathways as primary immune responses. Investigation into AcMNPV-induced apoptosis in the S. frugiperda cell line (Sf9) revealed that the Toll pathway activated the JNK via the TRAF6 (TNF receptor-associated factor 6) adapter. In addition, AcMNPV-induced the differential expression of several host-encoded microRNAs (miRNAs), with significant negative regulatory effects, on S. frugiperda antiviral immune genes. RNAi and miRNA-mimic mediated silencing of these genes resulted in increased AcMNPV proliferation. Our findings reinforce the potential of AcMNPV as a potent biocontrol agent and further our understanding of developing biotechnology-based targeted pest control agents.
Keyphrases
- induced apoptosis
- signaling pathway
- rna seq
- endoplasmic reticulum stress
- single cell
- oxidative stress
- genome wide
- immune response
- transcription factor
- bioinformatics analysis
- genome wide identification
- dna methylation
- diabetic rats
- dendritic cells
- cell proliferation
- gene expression
- risk assessment
- mass spectrometry
- drug delivery
- human health
- single molecule
- stress induced