Coumarin-Based Reversible Fluorescent Probe for Selective Detection of Cu2+ in Living Cells.
Nadeem AhmedWajeeha ZareenDi ZhangXiaopeng YangYong YePublished in: Journal of fluorescence (2020)
Copper ion plays an important role in many biological processes in human body. H2S is considered as the third gasses transmitter after carbon monoxide and nitric oxide. Here a novel ICT-based fluorescent ON-OFF-ON probe for Cu2+ and H2S detection was developed. Selectivity and sensitivity of probe was confirmed in aqueous Tris-HCl buffer (10 mM, pH 7.4, containing 90% acetonitrile). Probe DF-CU shows high selectivity over other analytes. The degree of fluorescence quenching is linearly associated with the concentration of Cu2+ (R2 = 0.9919). The limit of detection (LOD, calculated according to the 3σ/slope) for Cu2+ was 6.4 μM. Probe can work in almost all pH. The probe shows a very fast response to Cu2+ (within 10 s). Its response to copper ion could be reversed by H2S. The complex of probe with Cu2+ could be used for H2S detection. Furthermore, this ON-OFF-ON fluorescent probe successfully applied in the living cells for the detection of Cu2+ and H2S.