Construction of aptasensors for sensitive detection of 8-OH-dG based on a diffusion mediated electrochemiluminescence quenching effect.

Zhe FengRui-Juan ZhaoZe-Hua LuLi-Ping JiaRong-Na MaWei ZhangLei ShangQing-Wang XueHuai-Sheng Wang

Published in: Chemical communications (Cambridge, England) (2020)

A DNA immobilization-free ECL aptasensor was developed for the detection of 8-hydroxy-2'-deoxygunosine based on the diffusion mediated ECL quenching effect. This ECL aptasensor exhibited a high sensitivity and low detection limit by combining homogeneous DNA reaction with dual signal amplifications: target-induced multi-DNA release and Exo I-assisted target recycling.

Keyphrases

sensitive detection
loop mediated isothermal amplification
quantum dots
label free
energy transfer
circulating tumor
cell free
single molecule
oxidative stress
drug induced
high density