Dispersive Magnetic Solid-Phase Extraction as a Novelty Sample Treatment for the Determination of the Main Aflatoxins in Paprika.
María García-NicolásNatalia Arroyo-ManzanaresPilar ViñasPublished in: Toxins (2023)
Dispersive magnetic solid-phase extraction (DMSPE) technique is proposed as a new sensitive and effective sample treatment method for the determination of aflatoxins in paprika samples. DMSPE was followed by ultrahigh-performance liquid chromatography and high-resolution mass spectrometry detection (UHPLC-HRMS) using a non-targeted acquisition mode for the detection of main aflatoxins (aflatoxin G1, G2, B1 and B2) and derivatives. DMSPE was based on the use of magnetic nanocomposite coated with polypyrrole (PPy) polymer and the main experimental parameters influencing the extraction efficiency in adsorption and desorption steps have been studied and optimized. Analyses were performed using 250 µL magnetic PPy nanocomposite into the sample solution, adsorbing the analytes in 30 min and desorbing them with ethyl acetate (2 mL) in 15 min. The method has been validated, obtaining quantification limits between 3.5 and 4.7 µg kg -1 and recoveries between 89.5-97.7%. The high recovery rate, wide detection range and the use for the first time of the reusable Fe 3 O 4 @PPy nanomaterial in suspension for solid food matrices, guarantee the usefulness of the method developed for adequate control of aflatoxins levels in paprika. The proposed methodology was applied for the analysis of 31 samples (conventional and organic) revealing the absence of aflatoxins in the samples.
Keyphrases
- solid phase extraction
- molecularly imprinted
- liquid chromatography
- high resolution mass spectrometry
- tandem mass spectrometry
- high performance liquid chromatography
- ultra high performance liquid chromatography
- mass spectrometry
- liquid chromatography tandem mass spectrometry
- gas chromatography
- simultaneous determination
- gas chromatography mass spectrometry
- loop mediated isothermal amplification
- label free
- real time pcr
- high resolution
- ms ms
- drug delivery
- combination therapy
- replacement therapy