Establishment of fumonisin B 1 detection method for catalytic fluorescence detection of aptamer-regulated carbon dots.

Mycotoxin, common in agricultural products, is a small secondary metabolite with strong toxicity. Fumonisin B 1 (FB 1 ) is the most common and the most toxic. Establishing a rapid detection method is important for preventing and controlling FB 1 pollution. This study prepared carbon dots (CDs) from 2,2'-dithiosalicylic acid (DTSA). Tetramethylbenzidine (TMB) can be catalyzed to produce fluorescence by CDs, while FB 1 can adhere to the surface of CDs, decreasing fluorescence. Aptamer F10 of FB 1 combines with FB 1 attached to the surface of CDs to restore the catalytic ability of CDs and increase the fluorescence value. This method has good linearity in the FB 1 concentration range from 0 to 1.0 μg mL -1 . The standard curve was Y = -0.2512 x + 661.4, R 2 = 0.9903, the limit of detection (LOD) was 17.67 ng mL -1 and limit of quantitation (LOQ) was 53.55 ng mL -1 . The recovery of the corn sample was 89.83-98.62%, and the detection time was 30 min.