Zebrafish ambra1b knockout reveals a novel role for Ambra1 in primordial germ cells survival, sex differentiation and reproduction.

Camilla Maria FontanaFrancesca TerrinNicola FacchinelloGiacomo MeneghettiAlberto DinarelloLisa GambarottoAnnalisa ZuccarottoMicol CaichioloGinevra BroccaRanieri VerinFrancesca NazioOliana CarnevaliFrancesco CecconiPaolo BonaldoLuisa Dalla Valle

Published in: Biological research (2023)

By exploiting ambra1a and ambra1b knockout zebrafish lines, we prove the sub-functionalization between the two paralogous zebrafish genes and uncover a novel function of Ambra1 in the protection from excessive PGC loss, which seems to require binding with the CUL4-DDB1 complex. Both genes seem to play a role in the regulation of reproductive physiology.

Keyphrases

genome wide
induced apoptosis
genome wide identification
bioinformatics analysis
transcription factor
gene expression
oxidative stress
body mass index
cell death
binding protein
weight gain
dna methylation
genome wide analysis
endoplasmic reticulum stress