Convergent antibody evolution and clonotype expansion following influenza virus vaccination.
David ForgacsRodrigo B AbreuGiuseppe Andrea SauttoGreg A KirchenbaumElliott DrabekKevin S WilliamsonDongkyoon KimDaniel E EmerlingTed M RossPublished in: PloS one (2021)
Recent advances in high-throughput single cell sequencing have opened up new avenues into the investigation of B cell receptor (BCR) repertoires. In this study, PBMCs were collected from 17 human participants vaccinated with the split-inactivated influenza virus vaccine during the 2016-2017 influenza season. A combination of Immune Repertoire Capture (IRCTM) technology and IgG sequencing was performed on ~7,800 plasmablast (PB) cells and preferential IgG heavy-light chain pairings were investigated. In some participants, a single expanded clonotype accounted for ~22% of their PB BCR repertoire. Approximately 60% (10/17) of participants experienced convergent evolution, possessing public PBs that were elicited independently in multiple participants. Binding profiles of one private and three public PBs confirmed they were all subtype-specific, cross-reactive hemagglutinin (HA) head-directed antibodies. Collectively, this high-resolution antibody repertoire analysis demonstrated the impact evolution can have on BCRs in response to influenza virus vaccination, which can guide future universal influenza prophylactic approaches.
Keyphrases
- single cell
- high throughput
- healthcare
- rna seq
- high resolution
- acute lymphoblastic leukemia
- heavy metals
- high throughput sequencing
- mental health
- induced apoptosis
- tyrosine kinase
- endothelial cells
- emergency department
- chronic myeloid leukemia
- cell cycle arrest
- mass spectrometry
- health insurance
- binding protein
- induced pluripotent stem cells
- adverse drug
- transcription factor
- current status
- electronic health record
- dna binding
- drug induced