Charge Reversal Polypyrrole Nanocomplex-Mediated Gene Delivery and Photothermal Therapy for Effectively Treating Papillary Thyroid Cancer and Inhibiting Lymphatic Metastasis.
Menghong XuDuo ZhaoYuwen ChenChaoyi ChenLulu ZhangLihong SunJing ChenQingshuang TangSuhui SunCheng MaXiaolong LiangShumin WangPublished in: ACS applied materials & interfaces (2022)
As a traditional treatment for papillary thyroid cancer (PTC), surgical resection of diseased tissues often brings lots of inconveniences to patients, and the tumor recurrence and metastasis are difficult to avoid. Herein, we developed a gene and photothermal combined therapy nanosystem based on a polypyrrole (Ppy)-poly(ethylene imine)-siILK nanocomplex (PPR ILK ) to achieve minimally invasive ablation and lymphatic metastasis inhibition in PTC simultaneously. In this system, gelatin-stabilized Ppy mainly acted as a photothermal- and photoacoustic (PA)-responsive nanomaterial and contributed to its well-behaved photosensitivity in the near-infrared region. Moreover, gelatin-stabilized Ppy possessed a charge reversal function, facilitating the tight conjunction of siILK gene at physiological pH (7.35-7.45) and its automatic release into acidic lysosomes (pH 4.0-5.5); the proton sponge effect generated during this process further facilitated the escape of siILK from lysosomes to the cytoplasm and played its role in inhibiting PTC proliferation and lymphatic metastasis. With the guidance of fluorescence and PA bimodal imaging, gene delivery and Ppy location in tumor regions could be clearly observed. As a result, tumors were completely eradicated by photothermal therapy, and the recurrences and metastases were obviously restrained by siILK.
Keyphrases
- cancer therapy
- lymph node
- minimally invasive
- photodynamic therapy
- signaling pathway
- drug delivery
- end stage renal disease
- genome wide
- copy number
- gene expression
- high resolution
- ejection fraction
- drug release
- chronic kidney disease
- blood brain barrier
- deep learning
- reduced graphene oxide
- molecularly imprinted
- prognostic factors
- atrial fibrillation
- combination therapy
- dna methylation
- gold nanoparticles
- neural network