A single cell atlas of sexual development in Plasmodium falciparum .
Sunil Kumar DoggaJesse C RopJuliana CudiniElias FarrAntoine DaraDinkorma T OuologuemAbdoulaye A DjimdeArthur M TalmanMara N K LawniczakPublished in: Science (New York, N.Y.) (2024)
The developmental decision made by malaria parasites to become sexual underlies all malaria transmission. Here, we describe a rich atlas of short- and long-read single-cell transcriptomes of over 37,000 Plasmodium falciparum cells across intraerythrocytic asexual and sexual development. We used the atlas to explore transcriptional modules and exon usage along sexual development and expanded it to include malaria parasites collected from four Malian individuals naturally infected with multiple P. falciparum strains. We investigated genotypic and transcriptional heterogeneity within and among these wild strains at the single-cell level, finding differential expression between different strains even within the same host. These data are a key addition to the Malaria Cell Atlas interactive data resource, enabling a deeper understanding of the biology and diversity of transmission stages.
Keyphrases
- plasmodium falciparum
- single cell
- rna seq
- high throughput
- escherichia coli
- mental health
- electronic health record
- gene expression
- transcription factor
- big data
- machine learning
- cell cycle arrest
- oxidative stress
- cell death
- mesenchymal stem cells
- deep learning
- cell therapy
- decision making
- high resolution
- heat shock protein