New Manual Quantitative Polymerase Chain Reaction Assay Validated on Tongue Swabs Collected and Processed in Uganda Shows Sensitivity That Rivals Sputum-based Molecular Tuberculosis Diagnostics.

Amy Steadman Alfred AndamaAlexey BallJob MukwatamunduKhushboo KhimaniTessa MochizukiLucy AsegeAlice BukirwaJohn Baptist KatoDavid KatumbaEsther KisakyeWilson MangeniSandra MwebeMartha NakayeIrene NassunaJustine NyawereAnnet NakaweesaCatherine CookPatrick Peter John Phillips Talemwa Nalugwa Christine M Bachman Fred Collins Semitala Bernhard H Weigl John T Connelly William Worodria Adithya Cattamanchi

Published in: Clinical infectious diseases : an official publication of the Infectious Diseases Society of America (2024)

Tongue swabs are a promising alternative to sputum for molecular diagnosis of TB, with sensitivity approaching sputum-based molecular tests. Our results provide valuable insights for developing successful tongue swab-based TB diagnostics.

Keyphrases

mycobacterium tuberculosis
pulmonary tuberculosis
cystic fibrosis
single molecule
high throughput
emergency department
mass spectrometry