Time-of-Sight Liquid Flow Measurements in the Low Nanoliters per Minute Scale.

We describe an affordable and robust measurement technique applicable to nanoscale liquid flow. The approach can provide good precision (<1% RSD) in the 1.5-15 nL/min flow range. The motion of a conductive/nonconductive immiscible segmental interface in a capillary is followed by an admittance detector. The conductive marker segment, e.g., a salt solution, is protected on both sides from the principal flow stream by immiscible guard segments, typically a fluorocarbon (FC) liquid, of significantly greater impedance. Fluorosilylation of the capillary ensures no other liquid film between the FC segments and the wall (perfect piston). A given interface/marker can typically be used only once in interface/front tracking systems. We overcome this by putting the sensor capillary in a valved configuration where the flow direction in the sensor is reversed before the guard/marker segments escape. Several strategies are possible to interpret flow rate from the sensor output, including the rate of the interface movement. During the measurement process, a change in this rate of movement can be detected in <1 s. Small temperature variations in the 25-35 °C range did not affect sensor behavior.