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OpenSTED: open-source dynamic intensity minimum system for stimulated emission depletion microscopy.

Stephanie A PierceJordan JacobelliKatherine S GivenWendy B MacklinJuliet T GopinathMark E SiemensDiego RestrepoEmily A Gibson
Published in: Neurophotonics (2024)
Our open-source DyMIN system is an inexpensive add-on to a conventional STED microscope that can reduce photobleaching. The system can significantly improve signal to noise for dynamic time-lapse STED imaging of live samples.
Keyphrases
  • high resolution
  • air pollution
  • high intensity
  • high throughput
  • high speed
  • optical coherence tomography
  • label free
  • fluorescence imaging
  • single cell